Meanwhile, the application of magnetic microsphere in biomedical field such as enzyme immobilization, cell separation, targeted medicine and diagnosis were narrated in detail. 同时着重阐述了磁性微球在固定化酶、向药物、胞分离、断等生物医学领域的应用。
The magnetic particle can produce selective adhesion to cell according to the wrapping layer, and this feature can be used for the cell separation, selection and medicine transportation, etc. 磁粒子根据所包裹的外层物质,可以与细胞产生选择性黏附,该特性可用于细胞分离、分选、药物运输等。
During the last 40 years, especially during the later 10 years, much progress has been made in artificial liver support system in experiment and clinical applications with the development of semipermeable membrane, capillary holly-fiber, hepatocyte cell separation and culture techniques. 经过40多年的研究,尤其是近10年来应用半透膜、中空纤维技术、肝细胞分离及培养等技术,人工肝支持系统得到了迅速发展,在动物实验及临床应用中取得了较好效果。
Blood cell separation, blood cell lysing and DNA purification were achieved on the microfluidic chip, respectively. 分别在芯片上实现了血细胞的分离、血细胞的破裂以及DNA的提纯。
Methods The umbilical cord blood AC133+ cells were separated and purified by magnetic-activated cell separation ( MACS) purification system and the purity of AC133+ selected cells were estimated by flow cytometry. 结果1、MACS?免疫磁珠分选系统分离、纯化脐带血AC133+细胞具有方法稳定可靠、纯度高的特点,ACl33+细胞的纯度在二次过柱后可达80%以上;
A Study of Epidermal Stem Cell Separation 表皮干细胞的快速高效分离
Comparative Study on the Cell Separation of the Secondary Xylem of the Annual Branch of Pear and Peach Tree 梨树和桃树新梢次生木质部细胞解离的比较研究
Synthesis Property Characterization of Core-shell Co@ SiO_2 Magnetic Nanoparticle and Its Biomedical Applications to Cell Separation and Cell Chip Co@SiO2核壳式纳米磁性粒子的合成、性质表征及在细胞分离和细胞芯片上的应用
Microfluidic Cell Separation Chips Based on Crossflow Filtration 基于错流过滤原理的微流控细胞分离芯片的研制
Conclusion Ultrasonic standing wave blood cell separation technology is safe and reliable, and is similar to centrifugal blood separation method. 结论:超声驻波血细胞分离法与离心分离法相似,安全、可靠。
The application of magnetic nano-material in the field of biomedicine is introduced from aspects of drug-target technology, cell separation, immunity analysis, absorption and immobility of enzyme and gene therapy and the problems are raised to be solved in its application. 从靶向药物载体技术,细胞分离技术,免疫分析,酶的吸附与固定作用和基因治疗几个方面简要分析磁性纳米材料在生物医学领域的应用及其发展过程中有待解决的问题。
Several kinds of preparation methods and the applied developments of magnetic polymer microsphere in cell separation, immobilized enzyme, DNA separation and medicine carrier are discussed in detail, and the prospects of magnetic polymer microsphere are also proposed. 在此基础上,对磁性高分子微球在细胞分离、固定化酶、靶向药物、核酸分离等领域的最新应用及存在的问题进行了分析,并指出了该领域今后的研究方向。
A New Endothelial Cell Separation Method 一种新的内皮细胞分离方法
Results: Patients with critical limb ischemia can be treated by bone marrow mobilization, peripheral blood stem cell separation and transplantation, which can significantly reduce rest pain, promote ulcer healing, and increase walk distance. 结果:采用骨髓动员外周血干细胞分离并移植治疗临床上严重肢体缺血的患者能明显减轻静息痛,促进溃疡愈合,增加步行距离。
The ES cell separation and cloning technique combined with genetic modification and the embryo engineering showed promising theoretical and practical values in animal growth and development model, the protection of endangered species, and establishment of advanced animal breeding systems etc.. ES细胞分离、克隆技术与遗传工程和胚胎工程相结合,对于阐明动物生长发育的基本规律、抢救和保护濒危动物遗传资源、建立先进的动物育种技术体系具有重大的理论意义和实践价值。
The sandwich-type microfluidic biochip for cell lysis potentially integrated with the biochip for cell separation and the biochip for deoxyribonucleic acid extraction could enable complete pretreatment of complex biologic samples, which can lay the foundation for realization of miniaturized total analysis system. 夹流式细胞破裂芯片具有与细胞分离芯片和脱氧核糖核酸(DNA)提取芯片相集成的潜力,可实现对复杂生物样品预处理操作,为实现微全分析系统打下良好基础。
The complete mathematical and physical study of the process provided a theoretical basis for the optimization of the subsequent studies of blood cell separation using ultrasonic standing waves. 从而实现了对这一过程完整的数学、物理描述,为超声驻波血细胞分离方法的工程优化奠定理论基础。
MFB has now been used in immobilized bioreactors, solid-liquid separation of fermentation, continuous affinity chromatography, cell separation in immunology and culture of plant cells and yeast. In this paper, the theory and characteristics of MFB are introduced. 磁场流化床在生化工程领域已经应用于固定化生物反应器,发酵液固液分离,连续亲合色谱,细胞分离,以及植物细胞和酵母细胞培养等过程。
Therefore, since 1970's, as a new type of functional material, it has gained broad applications in cell separation, protein purification, immunoassays, immobilized enzyme and target delivery of drug. 因此,自70年代以来,磁性微球作为一种新型的功能材料,在细胞分离、蛋白质提纯、免疫测定和靶向药物等诸多领域有着广阔的应用前景。
Chitinase inhibitor can prevent insect larve and pupa ecdysis and fungal cell separation through inhibiting chitinase activity. 几丁质酶抑制物可以通过抑制几丁质酶的活性,阻止昆虫幼虫和蛹蜕皮以及真菌子母细胞的分离而起到杀虫和抗真菌作用。
So the MPM are widely used in bio-medical and other fields, such as cell separation, protein purification, immunoassays, targeted drugs and diagnostics. 因为这些特性的存在使磁性高分子微球被广泛应用于生物化学和生物医学等领域,如细胞的分离、蛋白质的提纯、免疫测定、靶向药物和诊断等。
With the rapid development of the surgery, immunosuppressive drugs, organ preservation and cell separation techniques and immunological basis of transplantation, organ transplantation has become an effective treatment of organ failure and brought the new dawn to many terminally ill patients. 随着外科手术、免疫抑制药物、器官和细胞分离保存技术及移植免疫学基础的迅速发展,器官移植已经成为治疗器官功能衰竭的有效手段,给很多身患不治之症的患者带来了新生曙光。
Among that, magnetic materials as a common functional materials is more in a wide range of applications in biomedical and biotechnology, such as enzyme immobilization, protein and immune determination, the purification of RNA and DNA, cell separation and magnetic targeted drugs [ 6-9]. 而磁性材料作为一种常见的功能性材料在生物医学和生物技术方面更有着广泛的应用,如蛋白质和酶的固定化、免疫测定、RNA和DNA的提纯、细胞分离及磁性靶向药物。
【 Method 】 sterile conditions, take young SD rat femur, combined with whole bone marrow culture adherent cell separation and purification of MSCs. 【方法】无菌条件下取幼年SD大鼠的股骨,采用全骨髓培养结合细胞贴壁法分离纯化MSCs。
At the same time, a software was developed for cell motion measurements and a real time, on line monitoring system was realized during the cell separation processing. 同时,设计了用于细胞运动识别的相应软件,实现了在细胞分离过程中,对细胞的实时自动监控。
Conclusion: Enzyme digestion method is proved to be a better way for cell separation and culture in tissue engineering study, and the first to the fourth passage of SF cells can be used as seeding cells in the study of ACL tissue engineering. 结论:酶消化法分离培养SF细胞更适合于组织工程,SF细胞第一~第四代可作为构建组织工程化前交叉韧带的种子细胞。
Using this separation system, may cause to make the cost of blood stem cell separation reduce multiplied. 采用该分离系统后,可以使造血干细胞分离成本成倍地降低。
Functional magnetic polymer nano-material is an advanced material. It combines the properties of polymer materials, magnetic materials and nano-materials. It has been widely applied in cell separation, targeted cancer therapy, biological and medical field, especially in cancer treatment. 功能性高分子磁性纳米复合材料是一种新型材料,因其兼具高分子材料、磁性材料和纳米材料的性能,在细胞分离和肿瘤靶向治疗等生物、医学领域具有非常广泛的应用前景。